Gynaecological Cytology

Pap Smear/HPV (liquid)

Collected by physician or authorized personnel Using the Broom-Like Device: Obtain an adequate sampling from the cervix using a broom-like device. Insert the central bristles of the broom into the endocervical canal deep enough to allow the shorter bristles to fully contact the ectocervix. Push gently, and rotate the broom in a clockwise direction 5 times. Rinse the broom as quickly as possible into the PreservCyt Solution by pushing the broom into the bottom of the vial 10 times, forcing the bristles apart. As a final step, swirl the broom vigorously to further release material. Discard the collection device. Tighten the cap so that the torque line on the cap passes the torque line on the vial. Using the Endocervical Brush/Spatula Device: Obtain as adequate sampling from the ectocervix using a plastic spatula. Rinse the spatula as quickly as possible into the PreservCyt Solution vial by swirling the spatula vigorously in the vial 10 times. Discard the spatula. Obtain an adequate sample from the endocervix using and endocervical brush device. Insert brush into the cervix until only the bottom-most fibers are exposed. Slowly rotate ¼ or ½ turn in one direction. DO NOT OVER-ROTATE. Rinse the brush as quickly as possible in the PreservCyt Solution by rotationg the device in the solution 10 times while pushing against the PreservCyt vial wall. Swirl vigorously to further release material. Discard the brush. Tighten the cap so that the torque line on the cap passes the torque line on the vial. Record the patient’s name and J number on the vial. Record the patient information and medical history on the cytology requisition form.

Gynae samples collected using a broom-type or endocervical brush/plastic spatula combination collection devices. Specimen rinsed in prefilled 20 mL PreservCyt Solution vials.

Transport: Transport in PreservCyt to the Cytology Lab Storage: Room temperature

SMH

The patient should be tested 2 weeks after the first day of her menstrual period, and not when she is menstruating. The patient should not use vaginal medication, vaginal contraceptives, or douches during the 48 hours before the pap exam. Lubricant jellies should not be used to lubricate the speculum. Remove excess mucus or other discharge present before taking the sample. This should be gently removed with ring forceps holding a folded gauze pad. The cervix should not be cleaned by washing with saline or it may result in a relatively acellular specimen The sample should be obtained before the application of acetic acid. See Appendix B for Quick Gyn Reference Guide NOTE: for Gyn specimens, Ontario Cervical Screening Cytology Guidelines 2025, Ontario Cancer Care, recommends repeat Cytology after 3 months for time for cell regeneration.

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June 1, 2026